Proteomics (Core Resources)
- Our Services
Our Proteomics Core aids investigators and trainees with high performance computation in proteomics, as well as other “Omics” type applications needing efficient analysis of large-scale biological data sets.
Also, the Meharry Proteomics Core provides state of the art services, training and bioinformatics-driven data analysis to the Meharry translational research community.
It is our aim to keep this facility up to date computationally and intellectually with services to support education and research in proteomics, bioinformatics, computational biology, and systems biology.
The ability to incorporate proteomics into translational and clinical biomedical research is critical for the discovery of therapeutic interventions and high fidelity biomarkers of disease and response to therapy. This proposal request funds for developing a Meharry proteomics core facility.
The objective of this component of our MeTRC Program is to establish a Proteomics Core facility, which will serve a broad research community and will significantly enhance translational and clinical research at Meharry Medical College. The establishment of this facility is significant in developing novel diagnostics and therapeutic interventions for infectious diseases critical to woman's health.
The Specific Aims of the proposed activities are:
- To develop a Proteomics Core Facility to support Meharry's clinical and translational research activities, and;
- To develop collaborations and partnerships inside and outside of Meharry Medical College that accelerate clinical and translational research, with the common link being the reliance on state-of-the-art proteomics strategies.
Based on the interest of current faculty in this resource, and their successes to date with proteomics in exploratory studies with collaborators at other institutions, we are confident that this investment will yield significant returns of discovery and fulfil basic-translational-clinical collaborations locally and nationally.
The Meharry Medical College Proteomics Core is funded by resources provided by the Research Centers in Minority Institutions (RCMI) program of the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and by the Meharry Medical College Clinical and Translational Research Center (MeTRC). Without the support of these funding sources, we could not provide services to Meharry Medical College. Thank you!
Investigators, please cite this core appropriately in all abstracts, posters, papers and grant submissions:
“Experiments/Data analysis [include what you use] were performed through the use of the Meharry Medical College Proteomics Core, which is supported in part by the Research Centers in Minority Institutions Program of the NIH (RCMI: grant# G12RR003032) and by the Meharry Clinical and Translational Research Center (MeTRC: grant# 1 U54 RR026140-01).”
Please let us know when you publish, and if possible, send us an electronic reprint of the paper.
- ThermoFiinnegan LTQ Ion Trap Mass Spectrometer
- AB Sciex Tempo Nano-1Dplus HPLC
- AB Sciex 920 Autosampler with cooling module
- NanoSPRAY ESI source (James A. Hill Company)
- Thermo Scientific Xcalibur Mass Spectrometry Controller Software
- Thermo Scientific Proteome Discoverer Mass Informatics Platform Software
- MATLAB programming environment with bioinformatics, statistics and proteomics analysis suites
- CYTOSCAPE bioinformatics software for systems biology and biological pathway interaction network analysis
Services We Provide
The Meharry Proteomics Core was established in order to provide services, computer resources, support and training to the Meharry research community. Our goal is to ensure that these scientists across the college are able to effectively exploit existing and emerging computational technologies, especially related to “Omics” scale data-sets. We provide the following list of services in an attempt to reach these goals:
The proteomics core performs LC-MS/MS applications such as shotgun proteomic profiling and protein band identification. Additionally, we have partnerships with other regional Mass Spectrometry Cores to offer more proteomics applications such as 2D Difference In-Gel Electrophoresis (DIGE), Targeted Mass Spectrometry and Multidimensional LC-MS/MS (MudPIT).
Meharry Proteomics Core personnel provide consulting to enable investigators to frame research questions in proteomics terms and identify solutions to those questions. Core personnel are then available to facilitate these solutions.
- Sample Protocol (Protein in-gel Digestion Protocol)
Meharry Proteomics Core personnel enable grant submission by helping to identify proteomic and bioinformatic approaches to investigators’ research problems, framing the questions in “Omics” terms and providing letters of support that indicate sufficient expertise and tools exist to successfully complete the research indicated.
Whenever possible, data analysis is approached as a training opportunity. However, there are times when this is undesirable either due to the complexity of the data (and analysis options) or the wishes of the investigator. In these instances, we do perform data analysis but request the participation of the investigator, as an appropriate analysis requires a fundamental understanding of the biology and experiment, which can only be supplied by the person who has done the work.
Identify and make resources available
As proteomics/bioinformatics needs arise in the research community or in individual laboratories, core personnel will help identify and make available resources that will address these needs. We will also train persons to use these resources effectively.
Education and Training
Core personnel provide training primarily in one-on-one or small group settings. We also provide workshops for specific bioinformatics packages or approaches to larger groups as needs are identified that can be addressed in this format. Talks have been and continue to be presented to departments to inform investigators of the value of proteomics & bioinformatics to their research and the services that are provided by the Proteomics Core.
Other needs...contact us!
We are always happy to discuss science and in particular proteomics approaches to research questions. If we are unable to help you with your specific problem, we can usually refer you to someone who can.
Click here to access the submission form (will open in a new window)
· Core Resources Director, MeTRC
· Core Resources Managing Director, MeTRC
· Associate Managing Director, MeTRC